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CV Andreea CHIVA

Tear proteins electrophoresis

Orthopedic research

            The purpose of this site is to present the latest concepts about the electrophoresis used in laboratory medicine, with particular emphasis on Hyrys-Hydrasys and Capillarys SEBIA France automated systems.
            Many data presented are based on my own experience of almost 20 years in this area. Most of these results have been published or presented in international journals or conferences.
            The site contains two sections. One is intended for agarose gel electrophoresis and other for capillary electrophoresis, the most important technique currently used for the analysis of a large spectrum of proteins (serum and urinary proteins, immunoglobulins, hemoglobins, glycated hemoglobin, lipoproteins, isoenzymes, cerebrospinal proteins). The principle of these methods, their advantages and disadvantages, as well as the diagnostic and prognostic value are presented. Additionally, an illustrated guide, as well as a scientific support section (mini-review articles about the main topics in clinical electrophoresis research) will facilitate a good interpretation of electrophoresis results.
            A particular importance is given to agarose gel electrophoresis of proteins in biological fluids (saliva, joint fluid, tears), the only method able to separate and quantify the most important proteins using a small quantity of these fluids.
            In the same time, it is also presented the latest type of capillary electrophoresis with a special focus on diagnosis of hemoglobinopathies in newborns (using dried blood spot or core blood) and quantification of carbohydrate deficient transferrine CDT (a biomarker for alcohol abuse).
            The readers will also find a section in which two agarose gel electrophoresis used for the first time in my studies are presented: electrophoresis of tear proteins, as a new diagnostic tool for ocular and systemic diseases related to dry eye, as well as for high risk group for dry eye (computer users, contact lens wearers, cataract surgery) and electrophoresis of joint fluid, as a new prognostic tool for prosthetic performance in total joint arthroplasty.
            For supplementary information and professional advice a contact box is available for all those interested. ( dr.andreea.chiva@gmail.com )

Andreea Chiva
Senior Biochemist, PhD
University Emergency Hospital Bucharest Romania

Noutati

10.07.2015 - Capillarys Hb A1c- primul sistem din lume destinat determinarii hemoglobinei glicate Hb A1c prin electroforeza capilara

04.11.2014 - Electroforeza hemoglobinei la nou nascuti

10.06.2013 - Capillarys 2 Flex Piercing si Minicap Flex Piercing, primele sisteme automate de determinare a Hb A1c prin electroforeza capilara, acum disponibile in Romania

30.05.2012 - Linia pediatrica SEBIA CAPILLARYS de electroforeza a hemoglobinei la nou nascuti

03.05.2012 - Determinarea beta 2 transferinei in diagnosticul rinoreei cu LCR- comparatie intre diverse tehnici electroforetice in gel

05.04.2012 - Interferente si limite in interpretarea electroforezei proteinelor serice in diagnosticul gamapatiilor monoclonale

14.02.2012 - Determinarea CDT prin electroforeza capilara: comparatie cu HPLC, performante si caracteristici

08.09.2011 - Aplicatii ale kit-ului Hydragel HR SEBIA Franta pentru determinarea markerilor de degenerescenta neuronala in lichidul cefalorahidian

25.08.2011 - Determinarea hemoglobinei glicozilate Hb A1c prin electroforeza capilara sistemul Capillarys SEBIA Franta

09.08.2011 - Electroforeza capilara in sistem Capillarys SEBIA Franta vs HPLC in diagnosticul anomaliilor hemoglobinei

27.07.2011 - Determinarea cistatinei C, a transtiretinei si fractiunii Tau in LCR prin electroforeza de inalta rezolutie- Hydragel HR SEBIA Franta

22.07.2011 - Protocolul de diagnostic a sclerozei multiple- determinarea benzilor oligoclonale in LCR cu kit-ul Hydragel CSF

25.03.2011 - Ce alegem: electroforeza in gel sau electroforeza capilara?

07.02.2011 - Beta 2 transferina- marker esential in diagnosticul fistulelor de lichid cefalorahidian (LCR)

01.02.2011 - ASSIST si GEL SCAN- cea mai noua generatie de echipamente SEBIA pentru electroforeza in gel

11.10.2010 - Automate pentru electroforeza capilara SEBIA Franta- performante si caracteristici

09.07.2010 - Capillarys 2 Flex Piercing- cea mai noua generatie de electroforeza capilara SEBIA-Franta

25.06.2010 - Comparatie intre electroforeza capilara si cea clasica in gel

06.04.2010 - Premiera Europeana - Electroforeza proteinelor din lichidul articular

06.04.2010 - Premiera europeana - Electroforeza proteinelor lacrimale

11.02.2010 - Diagnosticul consumului cronic de alcool cu kit-ul Capillarys CDT Sebia Franta

03.02.2010 - Capillarys Neonat Hb-cel mai nou kit SEBIA-Franta

07.01.2010 - Capillarys2 si Minicap- cea mai noua generatie de automate de electroforeza capilara SEBIA-Franta

15.10.2009 - HYDRASYS ® 2-cea mai noua generatie de automate de electroforeza SEBIA-Franta

14.09.2009 - Determinarea electroforetica a LDL/HDL-colesterol- element de noutate in programul SEBIA

Actualitati Medicale

09.10.2012 - Diploma Salonului Universitar de Carte editia 2012

07.06.2012 - Electroforeza proteinelor lacrimale in sindromul de ochi uscat datorat factorilor de risc ambientali si profesionali

10.11.2011 - NOU! Aparitie editoriala

21.10.2011 - Hemoglobina glicozilata Hb A1c- test de diagnostic sau de monitorizare in diabetul zaharat tip II?

12.09.2011 - Electroforeza de inalta rezolutie a proteinelor din LCR in boala Parkinson

30.05.2011 - Actualitati in diagnosticul sclerozei multiple-Identificarea benzilor oligoclonale in LCR

02.05.2011 - Hemoglobina glicozilata-protocol de investigatie si factori de interferenta

10.01.2011 - Actualitati in monitorizarea accidentelor cardiovasculare postoperatorii in chirurgia ortopedica

12.10.2010 - Transferina deficienta in carbohidrati (CDT)- stuctura, metabolism si cinetica la consumatorii cronici de alcool

01.09.2010 - Dry eye and computer users

27.07.2010 - Investigatia proteinelor urinare- protocol de diagnostic

16.04.2010 - Actualitati in diagnosticul consumului cronic de alcool

10.02.2010 - Factori de interferenta in diagnosticul consumului cronic de alcool

21.12.2009 - Electroforeza proteinelor din lacrimi- element de noutate in diagnosticul afectiunilor oftalmologice

03.12.2009 - Rolul leptinei in patogenia artrozelor la pacientii obezi

26.11.2009 - Sindromul de ochi uscat si ecranele informatice

12.11.2009 - Homocisteina si pentozidina-factori de risc in osteoporoza

02.11.2009 - Cataracta, exercitiile fizice si ochiul uscat

29.10.2009 - Actualitati in osteoporoza

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